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Astrocytes, which cover more than 80% of the CNS cells, play a critical role in the maintenance of CNS homeostasis by secreting a large array of cytokines and chemokines. This prompted us to investigate the secretion of CXCL10, a CXC chemokine, and CCL2, a CC chemokine, from human astrocytes infected with two neurotropic flaviviruses: Japanese encephalitis virus (JEV), the causative agent of epidemic encephalitis and West Nile virus (WNV), the causative agent of sporadic encephalitis. We found that human astrocytes were highly permissive to JEV and WNV infections, the viral replication being accompanied by an upregulation of CXCL10 and CCL2 secretion. Interestingly, CXCL10 and CCL2 production were inversely regulated. These findings add new knowledge about the nature of a possible immune response directed against viruses in the CNS and the possible involvement of astrocytes in the immunopathogenesis of neuroinflammatory disease., but he’s not a long-term solution. Furthermore, the risk of growing stale with age is extremely high. Rinaldi’s “Met”, which was a benchmark for so much of the innovative, contemporary activity in opera over the past decade, is a good example of this. Written in the 1990s, the work is now regarded by many as stale, boring and impenetrable.
Let me briefly suggest a few additional possibilities for contemporary opera that would be less risky and therefore more exciting for the audience. It’s not really feasible to suggest a few good ideas, as the current castration of opera at the box office makes it hard to fund new work. There are loads of excellent examples of opera that could be adapted to suit the market, including Giancarlo Job and Eugenio Finardi’s “Struwwelpeter”, Michael Nyman’s “E ac619d1d87
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